海膽狀金奈米粒子/聚 ( ε-己內酯 ) /殼聚醣電紡奈米纖維用於抗菌活性包裝

Abstract

活性包裝為延長食物的保值期的方法之一，可藉由金屬奈米粒子的添加提升包裝之抗菌效益。本研究製備海膽狀金奈米粒子 (Urchin-like gold nanoparticles, UGNPs)，並將其沉積於聚 (ε-己內酯)( Poly-ε-caprolactone, PCL) / 殼聚醣 (chitosan, CS) 電紡P/C奈米纖維膜上。根據實驗結果得知左旋多巴 (levodopa, L-Dopa)為製備UGNPs之最佳還原劑，隨著L-Dopa：HAuCl4的莫耳比增加，奈米粒子表面成長的尖刺長度也增加；奈米纖維浸泡在奈米粒子溶液的時間長，負載於P/C奈米纖維膜上的UGNP含量增加。UGNPs的尖刺長度與沉積的UGNP含量提升對金黃色葡萄球菌 (Staphylococcus aureus, S. aureus) 和大腸桿菌 (Escherichia coli, E. coli) 之抑菌效果呈正相關。負載UGNP的P/C奈米纖維膜在熱穩定性、親水性、機械強度以及水氣滲透性的良好表現展現其具備作為抗菌活性包裝的潛力。

Active packaging is one of the strategies used to extend the shelf life of food products, and its antimicrobial efficacy can be enhanced by incorporating certain metal nanoparticles. In this study, the urchin-like gold nanoparticles (UGNPs) were synthesized and deposited onto poly(ε-caprolactone) / chitosan (PCL/CS) electrospun nanofiber membranes. Experimental results demonstrated that Levodopa (L-Dopa) served as the optimal reducing agent for UGNP synthesis. As the molar ratio of L-Dopa to HAuCl₄ increased, the spine length of the UGNPs also increased. Additionally, prolonged immersion time of the nanofibers in the UGNP solution led to a higher loading of UGNPs onto the PCL/CS membranes. Both the spine length and the deposited amount of UGNPs showed a positive correlation with the antibacterial efficacy against Staphylococcus aureus and Escherichia coli. The UGNP-loaded PCL/CS nanofiber membranes exhibited great mechanical strength, hydrophilicity, water vapor permeability, and thermal stability, demonstrating its potential for use as antimicrobial active packaging materials.